polyclonal rabbit anti-drd1 (Millipore)
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90
Structured Review
Millipore
polyclonal rabbit anti-drd1
Polyclonal Rabbit Anti Drd1, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+rabbit+anti-drd1/pmc06673263-122-0-8?v=Millipore
Average 90 stars, based on 1 article reviews
Polyclonal Rabbit Anti Drd1, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+rabbit+anti-drd1/pmc06673263-122-0-8?v=Millipore
Average 90 stars, based on 1 article reviews
polyclonal rabbit anti-drd1 - by Bioz Stars,
2026-07
90/100 stars
Images
1) Product Images from "Evidence That the BLOC-1 Protein Dysbindin Modulates Dopamine D 2 Receptor Internalization and Signaling But Not D 1 Internalization"
Article Title: Evidence That the BLOC-1 Protein Dysbindin Modulates Dopamine D 2 Receptor Internalization and Signaling But Not D 1 Internalization
Journal: The Journal of Neuroscience
doi: 10.1523/JNEUROSCI.1689-07.2007
Figure Legend Snippet: Effects of siRNA on cell surface levels of DRD2 in SH-SY5Y cells. A, Representative data from flow cytometric analysis of cell surface DRD2 expression. The overlaid histogram shows relative fluorescence intensities of DRD2 from 10,000 cells transfected with either random siRNA (black), DTNBP1 siRNA (green), or MUTED siRNA (pink). B, Changes in cell surface DRD2 expression by DTNBP1 siRNA or MUTED siRNA transfection. The geometric mean of the DRD2 fluorescence signal was obtained by flow cytometric analysis. Values are expressed as a percentage of random siRNA transfected cells. Error bars represent means ± SD (n = 3).
Techniques Used: Expressing, Fluorescence, Transfection
Figure Legend Snippet: Effects of dopamine stimulation on cell surface DRD1 or DRD2 levels in siRNA transfected rat primary neurons. The geometric mean of cell surface of DRD1 or DRD2 fluorescence was obtained by flow cytometric analysis. Values are expressed as a percentage of random siRNA transfected cells. Error bars represent means ± SD (n = 4).
Techniques Used: Transfection, Fluorescence
Figure Legend Snippet: Effects of dopamine stimulation on cell surface levels of DRD2 in DTNBP1 siRNA transfected SH-SY5Y cells. A, Representative data from flow cytometric analysis of cell surface DRD2. The overlaid histogram shows relative fluorescence intensities of DRD2 from cells treated with random siRNA (black), random siRNA plus dopamine (DA; green), DTNBP1 siRNA (pink), or DTNBP1 siRNA plus DA (blue). B, Dopamine-induced changes in cell surface DRD2 expression. The geometric mean of cell surface of DRD2 fluorescence was obtained by flow cytometric analysis. Values are expressed as a percentage of random siRNA transfected cells. Error bars represent means ± SD (n = 4). C–E, Representative confocal images of DRD2 expression in SH-SY5Y cells transfected with random siRNA (C, D) or DTNBP1 siRNA (E, F). C, E, Not treated. D, F, Treated with 10 μm DA for 30 min. Scale bar, 10 μm.
Techniques Used: Transfection, Fluorescence, Expressing
![A Autoradiography. Representative images of autoradiography of D 1 -TG and WT mouse heart sections labeled with 5 nM [ 3 H]SCH23390 (total binding, TB) or 5 nM [ 3 H]SCH23390 + 10 M butaclamol (unspecific binding, UB). B In situ hybridization. Expression of D 1 -dopamine receptor mRNA detected by DIG-labelled human D 1 -dopamine receptor antisense probes (A-X) and DIG-labelled mouse D 1 -dopamine receptor antisense probes (a-l). DIG-labeled sense probes were used as controls. (A-H): Low magnification microscopic images, scale bar = 500 µm. mRNA expression of D 1 -dopamine receptor was not detected in WT mouse atria (A) and ventricles (C); whereas expression of human D 1 -dopamine receptor mRNA was detected in D 1 -Tg mouse atria (E) and ventricles including interventricular septum (G). (B, D, F, H) are corresponding control sections hybridized with DIG-labeled sense probes. (I-X): High magnification microscopic images, scale bar = 50 µm. No or very scarce and weak D 1 -dopamine mRNA expression was detected in WT mouse atria (I) and interventricular septum (K), while abundant human D 1 -dopamine mRNA expression was detected in atriums (M), interventricular septum (O), and ventricle walls (Q, S). (J, L, N, P, R, T) are corresponding control sections hybridized with DIG-labeled sense probes. D 1 -dopamine mRNA expression was also detected in human cardiomyocytes (U, W); while no signal was seen in corresponding control sections hybridized with DIG-labeled sense probes (V, X). (a-d): Low magnification microscopic images, scale bar = 500 µm. (e-h): High magnification microscopic images, scale bar = 50 µm. Expression of mouse D 1 -dopamine receptor mRNA was not detected in WT mouse atria (a, e) and ventricles (c, g); (b, d, f, h) are corresponding control sections hybridized with DIG-labeled sense probes. Low magnification images (i and j, scale bar = 500 µm) and high magnification images (k and l, scale bar = 50 µm) showed that the mouse antisense probe also detected human D 1 -dopamine mRNA expression in D 1 -TG mouse interventricular septum (i, k), while the mouse sense probe (control) did not detect any specific signal (j, l). C : Histology. Histological staining of myocardial sections with hematoxylin-eosin (HE) or Masson-Goldner trichome (MG) staining revealed no apparent abnormalities or signs of fibrosis, neither in WT nor in D 1 -TG. Scale bar = 100 µm. D : PCR. Scatter plots with means for the expression of exogenous transgenic D 1 -dopamine receptors (human-DRD1, left hand side) or endogenous mouse D 1 -dopamine receptors. Ordinates indicate the amount of the mRNA as inferred from the amplification cycles (Cq). *indicates significant differences between the mRNA from the whole heart of D 1 -TG versus WT](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_6794/pmc11166794/pmc11166794__210_2023_2901_Fig2a_HTML.jpg)
